Botanical Garden of Vilnius University (Lithuanian: Vilniaus universiteto botanikos sodas) is a botanical garden situated in Vilnius, Lithuania.
The garden was established by professor J. E. Giliber (1741-1814) of Vilnius University in 1781. In 1832 the Vilnius University and Botanical Garden were closed. In 1919, the Botanical Garden was started in a new location. In 1975 territory of the garden was expanded, and since then the main part of the garden is in Kairėnai (address is: Vilnius, Kairėnų Street, 43), which is situated in Antakalnis elderate of Vilnius. There is also a department of the garden in Vingis Park.